pcdna 3.1(+)图谱及序列

Alt Names              pcDNA3.1+, pcDNA3.1  Source/Vendor           Invitrogen  Plasmid Type            Mammalian  Viral/Non-viral           Nonviral  Stable/Transient          Transient  Constitutive/Inducible     Constitutive  Promoter                CMV  Expression Level          High  Plasmid Size              5428  Sequencing Primer         T7 Fwd  

Sequencing Primer Sequence   5'd[TAATACGACTCACTATAGGG]3'  Bacterial Resistance       Ampicilin  Mammalian Selection      G418, neo  

Notes Differs from other pcDNA3.1 in drug resistance; +/- refers to orientation of f1 ori.  Catalog Number V790-20  

1 GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATC  51 TGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTT  101 GGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAG  151 GCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCG  201 CTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGAC  251 TAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATA  301 TGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCG  351 CCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGT  401 AACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGT  451 AAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCC  501 CCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTA  551 CATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCA  601 TCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGA  651 TAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAA  701 TGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTA  751 ACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAG  801 GTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTG  851 GCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGC  901 GTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCACTAGTCCAGTGTGGT  951 GGAATTCTGCAGATATCCAGCACAGTGGCGGCCGCTCGAGTCTAGAGGGC  1001 CCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCA  1051 TCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCAC  1101 TCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGA  1151 GTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGG  1201 GAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTAT  1251 GGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACG  1301 CGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGC  1351 GTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTT  1401 CCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATC  1451 GGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCC  1501 AAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATA  1551 GACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGAC  1601 TCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTT  1651 GATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCT  1701 GATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTT  1751 AGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCA  1801 TGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCA  1851 GCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGT  1901 CCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCC  1951 ATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAG  2001 GCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGG  2051 AGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCG  2101 GATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGAT  2151 GGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTA  2201 TGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGC  2251 TGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGT  

2301 GCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCAC  2351 GACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAA  2401 GGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCT  2451 CACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCG  2501 GCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAAC  2551 ATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAG  2601 GATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGC  2651 CAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATG  2701 GCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGA  2751 TTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGC  2801 GTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACC  2851 GCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCC  2901 TTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAA  2951 ATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCAC  3001 CGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCG  3051 GCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCAC  3101 CCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCAT  3151 CACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTT  3201 TGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCT  3251 AGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTG  3301 TTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTA  3351 AAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGC  3401 TCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATG  3451 AATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCG  3501 CTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCG  3551 GTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGA  3601 TAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACC  3651 GTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGAC  3701 GAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGG  3751 ACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTC  3801 CTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCG  3851 GGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGT  3901 GTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGC  3951 CCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTA  4001 AGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAG  4051 AGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACT  4101 ACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCA  4151 GTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCAC  4201 CGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAA  4251 AAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAG  4301 TGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAG  4351 GATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCT  4401 AAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGT  4451 GAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTG  

4501 ACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCC  4551 CCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTA  4601 TCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGC  4651 AACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAG  4701 TAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACA  4751 GGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGG  4801 TTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAG  4851 CGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCA  4901 GTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCAT  4951 GCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCAT  5001 TCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATA  5051 CGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGG  5101 AAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGAT  5151 CCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTT  5201 ACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGC  5251 AAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCC  5301 TTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGA  5351 TACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCAC  5401 ATTTCCCCGAAAAGTGCCACCTGACGTC