pGEX-6P-2大肠杆菌表达载体说明

编号 名称

北京华越洋VECT-­‐550 pGEX-­‐6P-­‐2

pGEX6P2载体基本信息

别名: pGEX-­‐6P-­‐2, p GEX6P2, p GEX 6P 2

质粒类型: 大肠杆菌蛋白表达载体

表达水平: 高拷贝

启动子: Tac

克隆方法: 多克隆位点，性内切酶

载体大小: 4985 b p

5' 测序引物及序列: pGEX5': GGGCTGGCAAGCCACGTTTGGTG

3' 测序引物及序列: pGEX3': C CGGGAGCTGCATGTGTCAGAGG

载体标签: N-­‐GST

载体抗性: Ampicillin 氨苄

备注: 复制子是pMB1

稳定性: 瞬时表达 Transient

组成型: 诱导表达

病毒/非病毒: 非病毒

pGEX6P2载体质粒图谱和多克隆位点信息

pGEX6P2载体简介

There are a range of Gluthatione Sepharose™ prepacked column and bulk media products available to purify GST Fusion proteins. For manual purification of sample volumes up to 600 µl use G ST S pinTrap™ microspin c olumns o r G ST M ultiTrap™ 4B 96-­‐well p lates. F or s ample v olumes between 600 µl and 10 ml use GST GraviTrap™ gravity flow column. Where sample volumes are above 10 m l, u se L abMate™ reservoir t ogether w ith G ST G raviTrap. A ll f ormats d escribed c an b e used f or p reparation o f s amples i n p arallel. I n a ddition G ST H iTrap™ 1 a nd 5 m l c olumns a nd G ST HiPrep™ FF 16/10 column are available for purification in a chromatography system such as the ÄKTA™ design s ystem. A lternatively, G luthatione S epharose b ulk m edia a re a vailable f rom 10 m l up to 500 ml. A GST Bulk Kit is also available combining 10 ml Gluthatione Sepharose 4B bulk medium a nd 5 e mpty c olumns w ith r equired b uffers.

pGEX系列载体：

pGEX-­‐1λT E coRI/BAP

pGEX-­‐2T

pGEX-­‐2TK

pGEX-­‐20T

pGEX-­‐3X

pGEX-­‐4T-­‐1

pGEX-­‐4T-­‐2

pGEX-­‐4T-­‐3

pGEX-­‐5X-­‐1

pGEX-­‐5X-­‐2pGEX-­‐5X-­‐3

pGEX-­‐6P-­‐1

pGEX-­‐6P-­‐2

pGEX-­‐6P-­‐3

pGEX-­‐KG

pGEX6P2载体序列

ORIGIN

1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG 61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT 121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC 181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA 241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC 361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC 421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA 481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC 541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG 661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT 721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA 781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT 841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 901 ATCCTCCAAA ATCGGATCTG GAAGTTCTGT TCCAGGGGCC CCTGGGATCC CCAGGAATTC 961 CCGGGTCGAC TCGAGCGGCC GCATCGTGAC TGACTGACGA TCTGCCTCGC GCGTTTCGGT 1021 GATGACGGTG AAAACCTCTG ACACATGCAG CTCCCGGAGA CGGTCACAGC TTGTCTGTAA 1081 GCGGATGCCG GGAGCAGACA AGCCCGTCAG GGCGCGTCAG CGGGTGTTGG CGGGTGTCGG 1141 GGCGCAGCCA TGACCCAGTC ACGTAGCGAT AGCGGAGTGT ATAATTCTTG AAGACGAAAG 1201 GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG 1261 TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA 1321 CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA 1381 AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA 1441 TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT 1501 CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG 1561 AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC 1621 GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT 1681 CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA 1741 GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT 1801 CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT 1861 GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT 1921 GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA 1981 CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA 2041 CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT 2101 GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC2161 GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT 2221 GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA 2281 CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT 2341 GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC 2401 GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG 2461 CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT 2521 CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG 2581 TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG 21 CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC 2701 TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA 2761 CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA 2821 GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC 2881 GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT 2941 GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG 3001 AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT 3061 TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC 3121 TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC 3181 GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA 3241 CACCGCATAA ATTCCGACAC CATCGAATGG TGCAAAACCT TTCGCGGTAT GGCATGATAG 3301 CGCCCGGAAG AGAGTCAATT CAGGGTGGTG AATGTGAAAC CAGTAACGTT ATACGATGTC 3361 GCAGAGTATG CCGGTGTCTC TTATCAGACC GTTTCCCGCG TGGTGAACCA GGCCAGCCAC 3421 GTTTCTGCGA AAACGCGGGA AAAAGTGGAA GCGGCGATGG CGGAGCTGAA TTACATTCCC 3481 AACCGCGTGG CACAACAACT GGCGGGCAAA CAGTCGTTGC TGATTGGCGT TGCCACCTCC 3541 AGTCTGGCCC TGCACGCGCC GTCGCAAATT GTCGCGGCGA TTAAATCTCG CGCCGATCAA 3601 CTGGGTGCCA GCGTGGTGGT GTCGATGGTA GAACGAAGCG GCGTCGAAGC CTGTAAAGCG 3661 GCGGTGCACA ATCTTCTCGC GCAACGCGTC AGTGGGCTGA TCATTAACTA TCCGCTGGAT 3721 GACCAGGATG CCATTGCTGT GGAAGCTGCC TGCACTAATG TTCCGGCGTT ATTTCTTGAT 3781 GTCTCTGACC AGACACCCAT CAACAGTATT ATTTTCTCCC ATGAAGACGG TACGCGACTG 3841 GGCGTGGAGC ATCTGGTCGC ATTGGGTCAC CAGCAAATCG CGCTGTTAGC GGGCCCATTA 3901 AGTTCTGTCT CGGCGCGTCT GCGTCTGGCT GGCTGGCATA AATATCTCAC TCGCAATCAA 3961 ATTCAGCCGA TAGCGGAACG GGAAGGCGAC TGGAGTGCCA TGTCCGGTTT TCAACAAACC 4021 ATGCAAATGC TGAATGAGGG CATCGTTCCC ACTGCGATGC TGGTTGCCAA CGATCAGATG 4081 GCGCTGGGCG CAATGCGCGC CATTACCGAG TCCGGGCTGC GCGTTGGTGC GGATATCTCG 4141 GTAGTGGGAT ACGACGATAC CGAAGACAGC TCATGTTATA TCCCGCCGTC AACCACCATC 4201 AAACAGGATT TTCGCCTGCT GGGGCAAACC AGCGTGGACC GCTTGCTGCA ACTCTCTCAG 4261 GGCCAGGCGG TGAAGGGCAA TCAGCTGTTG CCCGTCTCAC TGGTGAAAAG AAAAACCACC 4321 CTGGCGCCCA ATACGCAAAC CGCCTCTCCC CGCGCGTTGG CCGATTCATT AATGCAGCTG 4381 GCACGACAGG TTTCCCGACT GGAAAGCGGG CAGTGAGCGC AACGCAATTA ATGTGAGTTA 4441 GCTCACTCAT TAGGCACCCC AGGCTTTACA CTTTATGCTT CCGGCTCGTA TGTTGTGTGG 4501 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGGATTCAC 4561 TGGCCGTCGT TTTACAACGT CGTGACTGGG AAAACCCTGG CGTTACCCAA CTTAATCGCC 4621 TTGCAGCACA TCCCCCTTTC GCCAGCTGGC GTAATAGCGA AGAGGCCCGC ACCGATCGCC 4681 CTTCCCAACA GTTGCGCAGC CTGAATGGCG AATGGCGCTT TGCCTGGTTT CCGGCACCAG 4741 AAGCGGTGCC GGAAAGCTGG CTGGAGTGCG ATCTTCCTGA GGCCGATACT GTCGTCGTCC

4801 CCTCAAACTG GCAGATGCAC GGTTACGATG CGCCCATCTA CACCAACGTA ACCTATCCCA 4861 TTACGGTCAA TCCGCCGTTT GTTCCCACGG AGAATCCGAC GGGTTGTTAC TCGCTCACAT 4921 TTAATGTTGA TGAAAGCTGG CTACAGGAAG GCCAGACGCG AATTATTTTT GATGGCGTTG 4981 GAATT

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其他大肠杆菌表达载体：

pBV221 ptdTomato pET-­‐52b(+) pAmCyan

pDsRed-­‐Express2 pBV220 pCold-­‐GST pColdS-­‐SUMO

pCold T F pCold I V pCold I II pCold I I

pCold I pE-­‐SUMO pCold-­‐ProS2 pBAD102/D-­‐TOPO

pBAD202/D-­‐TOPO pACYC184 pBAD/Thio-­‐TOPO pBad/Myc-­‐His C

pBad/Myc-­‐His B pBad/Myc-­‐His A pBad/His C pBad/His B

pBad/His A pBAD-­‐TOPO pET-­‐23b(+) pET-­‐23a(+)

pET-­‐23c(+) pET-­‐23(+) pET-­‐12b(+) pET-­‐12c(+)

pET-­‐12a(+) pET-­‐11b(+) pET-­‐11a(+) pET-­‐11c(+)

pBad24 pQE-­‐82L pQE-­‐81L pQE-­‐80L

pQE-­‐32 pQE-­‐9 pQE-­‐16 pQE-­‐31

pQE-­‐60 pQE-­‐70 pQE-­‐40 pET-­‐51b(+)

pET-­‐50b(+) pET-­‐49b(+) pET-­‐48b(+) pET-­‐47b(+)

pET-­‐26b(+) pET-­‐32a(+) pET-­‐21b(+) pET-­‐22b(+)

pET-­‐14b pET-­‐16b pET-­‐15b pET-­‐19b

pET-­‐20b(+) pET-­‐21d(+) pET-­‐21c(+) pET-­‐21b(+)

pET-­‐21a(+) pET-­‐24a(+) pET-­‐24d(+) pET-­‐25b(+)

pET-­‐27b(+) pET-­‐28a(+) pET-­‐30a(+) pET-­‐42a(+)

pET-­‐43.1c(+) pET-­‐43.1b(+) pET-­‐43.1a(+) pET-­‐44a(+)

pET-­‐44c(+) pET-­‐46 E K/LIC pET-­‐37b(+) pTrcHis2 C

pTrcHis2 B pTrcHis2 A pET303/CT-­‐His pET302/NT-­‐His

pRSET-­‐CFP pRSET-­‐EmGFP pRSET-­‐BFP pGFPuv

pET300/NT-­‐DEST pET301/CT-­‐DEST pGEM-­‐T pBad43

pGEX-­‐4T-­‐3 pGEX-­‐5X-­‐2 pBlueScript S K(+) pG-­‐Tf2

pG-­‐KJE8 pGro7 pET-­‐SUMO pSE380

pET-­‐17b pET102/D-­‐TOPO pCDFDuet-­‐1 pMAL-­‐p5x

pTf16 pET-­‐28c(+) pBluescript I I S K(+) pET-­‐30b(+)

pSUMO pProEX H Tc pProEX H Tb pProEX H Ta

pKD3 pKD13 pKD46 pTYB1

pTYB2 pTWIN2 pBluescript I I K S(-­‐) pTYB12

pMAL-­‐p5e pACYCDuet-­‐1 pEGM-­‐11ZF(+) pEGM-­‐7ZF(+)

PinPoint X a-­‐3 PinPoint X a-­‐2 PinPoint X a-­‐1 pSP73

pSP pTWIN1 pTYB11 pTXB1

pET-­‐5b(+) pBad/gIII C pBad/gIII B pBad/gIII A

pET-­‐5a(+) pMal-­‐p4X pMal-­‐p2G pkk223-­‐3

pkk232-­‐8 pCYB1 pEZZ18 pBAD18

pMAL-­‐c5x pMal-­‐p2E pMal-­‐p2X pET-­‐44 E K/LICpET-­‐43.1 E K/LIC pET-­‐41 E K/LIC pMal-­‐c4X pTrcHis B pET-­‐31b(+) pET-­‐3b(+) pET-­‐41a(+) pGEX-­‐3X pGEX-­‐4T-­‐2 pETDuet-­‐1 pGEX-­‐4T-­‐1 pTrc99a pET-­‐28b(+) pET-­‐His pALEX a,b,c pACYC177 pBR322 pKD4 pKD20 pMXB10 pEcoli-­‐6xHN-­‐GFPuv pKJE7 pRSET B pGEX-­‐KG pGEX-­‐2T pRSFDuet-­‐1 pCOLADuet-­‐1 pTrcHis C pTrcHis A pET-­‐41b(+) pET-­‐42b(+) pET-­‐3a(+) pGEX-­‐6P-­‐3 pGEX-­‐6P-­‐2 pGEX-­‐6P-­‐1 pGEX-­‐5X-­‐3 pGEX-­‐5X-­‐1 pGEX-­‐2TK pRSET A pMal-­‐c2G pMal-­‐c2E pMal-­‐c2X pRSET C pQE-­‐30

pET-­‐45b(+) pET-­‐44b(+) pET-­‐42c(+) pET-­‐41c(+) pET-­‐40b(+) pET-­‐33b(+) pET-­‐39b(+) pET-­‐32 E K/LIC pET-­‐32 X a/LIC pET-­‐32c(+) pET-­‐32b(+) pET-­‐30 X a/LIC pET-­‐30 E K/LIC pET-­‐30c(+) pET-­‐29c(+) pET-­‐29b(+) pET-­‐29a(+) pET-­‐24c(+) pET-­‐24b(+) pET-­‐24(+) pET-­‐23d(+) pET-­‐11d(+) pBad33