10mM PBS 配方

来源：动视网责编：小OO 时间：2025-09-23 10:48:02

10mM PBS 配方

1XPhosphateBufferedSaline(PBSBuffer)Recipe1.Dissolvethefollowingin800mldistilledH2O.▪8gofNaCl▪0.2gofKCl▪1.44gofNa2HPO4▪0.24gofKH2PO4▪2.AdjustpHto7.4withHCl.3.Adjustvolumeto1LwithadditionaldistilledH2O.4.Sterilizebyautoclaving.PeoplecallthisPBS10mM,b

推荐度：

点击下载本文 文档为doc格式

导读1XPhosphateBufferedSaline(PBSBuffer)Recipe1.Dissolvethefollowingin800mldistilledH2O.▪8gofNaCl▪0.2gofKCl▪1.44gofNa2HPO4▪0.24gofKH2PO4▪2.AdjustpHto7.4withHCl.3.Adjustvolumeto1LwithadditionaldistilledH2O.4.Sterilizebyautoclaving.PeoplecallthisPBS10mM,b

1X Phosphate Buffered Saline (PBS Buffer) Recipe

1.Dissolve the following in 800ml distilled H2O.

▪8g of NaCl

▪0.2g of KCl

▪1.44g of Na2HPO4

▪0.24g of KH2PO4

▪

2.Adjust pH to 7.4 with HCl.

3.Adjust volume to 1L with additional distilled H2O.

4.Sterilize by autoclaving.

People call this PBS 10mM, because the important component for buffer is Na2HPO4

137 mM NaCl

2.7 mM KCl

10 mM Na2HPO4

2 mM KH2PO4

Ionic strength:

10mM PBS 配方

1XPhosphateBufferedSaline(PBSBuffer)Recipe1.Dissolvethefollowingin800mldistilledH2O.▪8gofNaCl▪0.2gofKCl▪1.44gofNa2HPO4▪0.24gofKH2PO4▪2.AdjustpHto7.4withHCl.3.Adjustvolumeto1LwithadditionaldistilledH2O.4.Sterilizebyautoclaving.PeoplecallthisPBS10mM,b

推荐度：

点击下载本文 文档为doc格式

热门焦点

10mM PBS 配方

10mM PBS 配方

10mM PBS 配方

最新推荐

猜你喜欢

热门推荐