各种组分名称 | 各种凝胶体积所对应的各种组分的取样量 | |||||||
5ml | 10ml | 15ml | 20ml | 25ml | 30ml | 40ml | 50ml | |
6% Gel H2O 30% Acrylamide 1.5M Tris-HCl(pH8.8) 10% SDS 10% 过硫酸铵 TEMED | 2.6 1.0 1.3 0.05 0.05 0.004 | 5.3 2.0 2.5 0.1 0.1 0.008 | 7.9 3.0 3.8 0.15 0.15 0.012 | 10.6 4.0 5.0 0.2 0.2 0.016 | 13.2 5.0 6.3 0.25 0.25 0.02 | 15.9 6.0 7.5 0.3 0.3 0.024 | 21.2 8.0 10.0 0.4 0.4 0.032 | 26.5 10.0 12.5 0.5 0.5 0.04 |
8% Gel H2O 30% Acrylamide 1.5M Tris-HCl(pH8.8) 10% SDS 10% 过硫酸铵 TEMED | 2.3 1.3 1.3 0.05 0.05 0.003 | 4.6 2.7 2.5 0.1 0.1 0.006 | 6.9 4.0 3.8 0.15 0.15 0.009 | 9.3 5.3 5.0 0.2 0.2 0.012 | 11.5 6.7 6.3 0.25 0.25 0.015 | 13.9 8.0 7.5 0.3 0.3 0.018 | 18.5 10.7 10.0 0.4 0.4 0.024 | 23.2 13.3 12.5 0.5 0.5 0.03 |
10% Gel H2O 30% Acrylamide 1.5M Tris-HCl(pH8.8) 10% SDS 10% 过硫酸铵 TEMED | 1.9 1.7 1.3 0.05 0.05 0.002 | 4.0 3.3 2.5 0.1 0.1 0.004 | 5.9 5.0 3.8 0.15 0.15 0.006 | 7.9 6.7 5.0 0.2 0.2 0.008 | 9.9 8.3 6.3 0.25 0.25 0.01 | 11.9 10.0 7.5 0.3 0.3 0.012 | 15.9 13.3 10.0 0.4 0.4 0.016 | 19.8 16.7 12.5 0.5 0.5 0.02 |
12% Gel H2O 30% Acrylamide 1.5M Tris-HCl(pH8.8) 10% SDS 10% 过硫酸铵 TEMED | 1.6 2.0 1.3 0.05 0.05 0.002 | 3.3 4.0 2.5 0.1 0.1 0.004 | 4.9 6.0 3.8 0.15 0.15 0.006 | 6.6 8.0 5.0 0.2 0.2 0.008 | 8.2 10.0 6.3 0.25 0.25 0.01 | 9.9 12.0 7.5 0.3 0.3 0.012 | 13.2 16.0 10.0 0.4 0.4 0.016 | 16.5 20.0 12.5 0.5 0.5 0.02 |
15% Gel H2O 30% Acrylamide 1.5M Tris-HCl(pH8.8) 10% SDS 10% 过硫酸铵 TEMED | 1.1 2.5 1.3 0.05 0.05 0.002 | 2.3 5.0 2.5 0.1 0.1 0.004 | 3.4 7.5 3.8 0.15 0.15 0.006 | 4.6 10.0 5.0 0.2 0.2 0.008 | 5.7 12.5 6.3 0.25 0.25 0.01 | 6.9 15.0 7.5 0.3 0.3 0.012 | 9.2 20.0 10.0 0.4 0.4 0.016 | 11.5 25.0 12.5 0.5 0.5 0.02 |
各种组分名称 | 各种凝胶体积所对应的各种组分的取样量 | |||||||
1ml | 2ml | 3ml | 4ml | 5ml | 6ml | 8ml | 10ml | |
H2O 30% Acrylamide 1.0M Tris-HCl(pH6.8) 10% SDS 10% 过硫酸铵 TEMED | 0.68 0.17 0.13 0.01 0.01 0.001 | 1.4 0.33 0.25 0.02 0.02 0.002 | 2.1 0.5 0.38 0.03 0.03 0.003 | 2.7 0.67 0.5 0.04 0.04 0.004 | 3.4 0.83 0.63 0.05 0.05 0.005 | 4.1 1.0 0.75 0.06 0.06 0.006 | 5.5 1.3 1.0 0.08 0.08 0.008 | 6.8 1.7 1.25 0.1 0.1 0.01 |