PBS缓冲液

0.2M NaH2PO4：称取 31.2g NaH2PO4-2H2O，溶于1000ml 水.

各种浓度PB(pH=7.4)的配制：先配 0.2M PB (pH=7.4，100ml)：取19ml 0.2mol...

PBS是最普遍不过的实验室缓冲液，但其配方各异。以下是总结： 

母液的配制： 

0.2M Na2HPO4：称取 71.6g Na2HPO4-12H2O，溶于 1000ml 水

0.2M NaH2PO4：称取 31.2g NaH2PO4-2H2O，溶于1000ml 水

各种浓度PB(pH=7.4)的配制：

先配 0.2M PB (pH=7.4，100ml)：取19ml 0.2mol/L的 NaH2PO4， 81ml 0.2mol/L 的 Na2HPO4, 即可。

然后只需将0.2M PB (pH=7.4)按相应比例适当稀释即可，如：

0.1M PB（PH=7.4）：取 500ml 0.2M PB，加水稀释至 1000ml 即可。

0.01M PB （PH=7.4）：取50ml 0.2M PB，加水稀释至 1000ml 即可。

0.02M PB （PH=7.4）：取100ml 0.2 M PB，加水稀释至 1000ml 即可。 

若需要NaCl的话，加入NaCl至0.9%（g/100ml）即可。 

另：其它各种另 PH值的 0.2M PB（100ml)配方：

pH       0.2M NaH2PO4（ml）         0.2M Na2HPO4（ml）

5.7             93.5                          6.5

5.8             92                             8            

5.9             90                             10

6.0             87.7                          12.3

6.1             85                             15

6.2             81.5                          18.5

6.3             77.5                          22.5

6.4             73.5                          26.5

6.5             68.5                          31.5

6.6             62.5                          37.5

6.7             56.5                          43.5

6.8             51                            49

6.9              45                           55

7.0              38                           62

7.1              33                           67

7.2             28                            72

7.3              23                           77

7.4              19ml                         81ml

7.5              16                           84

7.6              13                           87

7.7             10.5                          0.5

7.8              8.5                          91.5

7.9             7                              93

8.0             5.3                           94.7